The objective of the study was to determine the relationship between phenols and graft incompatibility in Uapaca kirkiana. Phenol quantification and identification were carried out using Folin-Ciocalteau reagent procedure fluorescence microscopy and reverse phase high performance liquid chromatograph (RP-HPLC) above below and at the graft union. Results showed no vascular cambium continuity above the scion/stock unions. Significant differences in total soluble phenols and cell wall bound phenols were obtained. Fluorescence microscope indicated the presence of flavonoids and other polymers above the union. The RP-HPLC identified ferulic acid as a major phenol component found in U. kirkiana plant cells and responsible for wood discolouration. High phenol concentrations were obtained in less compatible combinations than in compatible combinations. High peaks of -coumaric acid were obtained above the union. It is concluded that phenols especially -coumaric acids and flavonoids caused poor callus formation at the union and hence implicated in graft incompatibility.
DOI:
http://dx.doi.org/10.1016/j.scienta.2008.03.031Altmetric score:
Dimensions Citation Count:
Publication year
2008
Authors
Mng'omba, S.A.; du, Toit.E.S.; Akinifesi, F.K.
Keywords
Callus, Euphorbiaceae, Exudates, Fields, Secondary metabolites
