The ability to precisely trap, transport and manipulate micrometer-sized objects is very important in microfluidic applications. Caenorhabditis elegans (C. elegans) is one of the most commonly used animals in biological and medical research due to the small size, well-mapped neuronal system, transparent body and the ease to culture. In many studies, worms need to be selected, transported and immobilized for experiments and observation. In this study, we demonstrate that acoustic radiation forces, including Primary Radiation Force (PRF) and Secondary Radiation Force (SRF), can be used to precisely manipulate C. elegans. Due to the non-contact and no-electrical-field nature, we believe that the technologies developed here provide novel ways of manipulating of C. elegans, as well as potentially any other biological samples.
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