Identification and characterization of nuclear cleaved amplified polymorphic sequence (CAPS) loci in Irvingia gabonensis and I. wombolu, indigenous fruit trees of west and central Africa

Irvingia gabonensis and I. wombolu (Irvingiaceae) are economi-cally important fruit trees native to moist tropical forest inwest and central Africa (Harris 1996). Currently, both speciesare the subject of intensive research due to their potential forincorporation in agroforestry systems and concerns over highrates of deforestation in their native ranges (Ladipo et al.1996). To inform evaluation and conservation programs, it isnecessary to understand the partitioning of genetic diversityin both species. Techniques such as RAPDs (Williams et al.1990) are often applied to previously unstudied taxa becauseno sequence information is required. However, these markersare dominant and of limited utility for studying the reproduc-tive ecology of species (Dawson et al. 1995). In addition, suchmarkers do not provide phylogenetic information whichoffers extra insights into contemporary patterns of geneticvariation within taxa (Avise 1994) and can provide evidenceof origin and ancient gene flow. Here, we describe the devel-opment of anonymous, nuclear, cleaved amplified polymor-phic sequences (CAPS), which have been successfully appliedto a range of phylogeographic and population genetic ques-tions (e.g. Avise 1994), for I. gabonensis and I. wombolu

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DOI:

https://doi.org/10.1046/j.1365-294x.1998.00520.x

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