an efficient culture method for bovine mammary epithelial cells, and to research the induction effect of hormones and cytokines on -casein (CNS 2) mRNA expression Bovine mammary epithelial cells were cultured by tissue mass culture method. And mechanical disruption method, respectively. Cells were treated according to different sensitivity to trypsin of fibroblasts and bovine mammary epithelial cells. The growth curve of purified cells was detected by cell counting assay method. The expression of keratin 18 was detected by immunofluorescent histochemistry staining method . CNS 2 mRNA expression induced by 8 kinds of hormones and cytokines combination mediums was measured by real time quantified PCR (RT-qPCR). The results showed that vigorous bovine mammary epithelial cells could be obtained by mechanical disruption method. Cell migration speed of mechanical The expression of cytokeratin 18 was found in purified cells and the 20th generation of cells. The supplementation of 100 ng / mL insulin-like growth factor (IGF-) significantly improved CNS 2 mRNA expression in mammary epithelial cells (P
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