Drought resistance has become a hot issue in rice scientific research. By Agrobacterium tumefaciensmediated,IPT gene was transformed to rice IR64 variety. 254 regenerated were obtained, and PCR and Southernblotting analyzsis were carried on them to confirm integration of target gene in the transgenic plant. Two transgenicsingle copy events and one wild type were treated with drought stress at flowering stage. Under the same drought stresscondition, the cytokinins and chlorophyll contents of transgenic plants were obviously higher than that of the wildtype. While the leaf temperature was slightly lower than that of the wild type. The stem and leaf senescence oftransgenic plant was delayed. These result shows that IPT gene has positive significance on studying drought tolerancebreeding and its mechanism.
Publication year
2022
Authors
Maimaiti Y; Ye-hui X; Maihemutijiang M
Language
English
