Limited access to high quality planting material is a major factor constraining productivity of pyrethrum in Rwanda. The objective of this study was to develop an efficient protocol for regeneration and mass propagation of high yielding and high pyrethrin content pyrethrum cultivars using tissue culture techniques. Nodal explants from in vitro grown pyrethrum plantlets were cultured onto Murashige and Skoog (MS) media supplemented with different concentrations of cytokinins 2-isopentyl adenine (2iP), Benzylaminopurine (BAP), Kinetin (KIN), Thidiazuron (TDZ), cysteine, 100 mg/l- Inositol, 2 % sucrose and gelled with 7.0 % agar. Rooting was evaluated using half strength MS media supplemented with Indole-3-butyric acid (IBA), Indole-3-acetic acid (IAA), 1-naphtyleneacetic acid (NAA) and Dichlorophenoxy-acetic acid (2,4-D). Media without growth regulators was used as control. Results showed that there were significant (p
Publication year
2013
Authors
Lindiro C; Kahia, J.; Asiimwe T; Mushimiyimana I; Waweru B; Kouassi M; Koffi E; Kone S; Sallah Y P
Language
English
Keywords
chrysanthemum cinerariaefolium, germplasm, plantlets, inoculation
Geographic
Rwanda
