Randomly amplified polymorphic DNA (RAPD), and a mitochondrial marker based on amplification of the V7 region of the mitochondrial small ribosomal RNA (srRNA) gene, were used to partition genetic variation within a single population of Gliricidia sepium sampled from Guatemala. Seventeen per cent of the variation detected with RAPDs was partitioned among subpopulations and indicated a greater level of discrimination than previously detected with isozymes. Cluster analysis indicated a direct relationship between this variation and the geographical distance between subpopulations. A polymorphism identified within the maternally inherited mitochondrial V7 srRNA product, which relied on digestion with restriction endonucleases, confirmed the genetic subdivision identified with RAPDs, and suggested a relatively limited role for seed in gene dispersal.
DOI:
https://doi.org/10.1038/hdy.1995.2Altmetric score:
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Publication year
1995
Authors
Dawson I A K; Simons A J; Waugh R; Powell W
Language
English
Keywords
mitochondrial genetics, genetic markers, ribosomal rna, geography, isoenzymes, genetic variation, dna, genetics, multipurpose trees
