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Nous fournissons des preuves et des solutions concrètes pour transformer l’utilisation des terres et la production alimentaire : conserver et restaurer les écosystèmes, répondre aux crises mondiales du climat, de la malnutrition, de la biodiversité et de la désertification. En bref, nous améliorons la vie des populations.

CIFOR-ICRAF publie chaque année plus de 750 publications sur l’agroforesterie, les forêts et le changement climatique, la restauration des paysages, les droits, la politique forestière et bien d’autres sujets encore, et ce dans plusieurs langues. .

CIFOR-ICRAF s’attaque aux défis et aux opportunités locales tout en apportant des solutions aux problèmes mondiaux concernant les forêts, les paysages, les populations et la planète.

Nous fournissons des preuves et des solutions concrètes pour transformer l’utilisation des terres et la production alimentaire : conserver et restaurer les écosystèmes, répondre aux crises mondiales du climat, de la malnutrition, de la biodiversité et de la désertification. En bref, nous améliorons la vie des populations.

CIFOR–ICRAF publishes over 750 publications every year on agroforestry, forests and climate change, landscape restoration, rights, forest policy and much more – in multiple languages.

CIFOR–ICRAF addresses local challenges and opportunities while providing solutions to global problems for forests, landscapes, people and the planet.

We deliver actionable evidence and solutions to transform how land is used and how food is produced: conserving and restoring ecosystems, responding to the global climate, malnutrition, biodiversity and desertification crises. In short, improving people’s lives.

A novel regeneration system for Tamarillo (Cyphomandra betacea) based on organogenesis from juvenile tissues

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Cyphomandra betacea (Cav.) is commonly known as Tamarillo or tree tomato. This species is mainly used for its edible fruits which have a high nutritional value and contain relatively high amounts of proteins, vitamins B6, C, E, and provitamin A. The cultivation of Tamarillo in Rwanda is facing major challenges caused mainly by viral diseases such as Tamarillo mosaic virus (TaMV). These diseases are difficult to control and are transferred through vegetative propagation, often resulting in heavy productivity losses and poor-quality fruits. Thus, this study was conducted to evaluate the possibility of using tissue culture as an alternative propagation method. Tamarillo seeds were sterilized using a commercial bleach and germinated in vitro to get clean starting explants. Explants (hypocotyls, leaves, and roots) were cultured on semisolid Murashige and Skoog (MS) media supplemented with 6-benzylaminopurine (BA), N6-2-isopentyl adenine (2iP), 6-furfurylaminopurine (kinetin) evaluated at 5, 10, 20, 40 µM, and thidiazuron (TDZ), evaluated at 0.1, 0.5. 1.0 1.5 µM in separate experiments. Data were collected on the number of microshoots and roots 2 months after culture and analyzed using the Statistical Software for Social Sciences (SPSS) Software version 8. The results showed that the growth regulators evaluated had a significant (P 0.05) effect on plantlet regeneration from leaf and hypocotyl explants. The media supplemented with BA 40 M was the most effective in inducing multiple shoots from leaf explants producing 4.67 ± 0.15 shoots per explant. Root explants showed the least morphogenic responses for all the parameters evaluated. The regenerated plantlets were transplanted to the greenhouse and a survival rate of 90% was recorded. During this study, a simple, reproducible, single-step protocol was developed. These results would be useful for mass propagation of Tamarillo.

DOI:
https://doi.org/10.21273/HORTSCI.50.9.1375
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