The document summarizes research being conducted on Warburgia ugandensis, an important agroforestry tree in East Africa. The research aims to 1) identify genes and genomic regions related to active compounds in W. ugandensis, 2) develop a marker system for selecting genotypes with high anti-malarial activity, and 3) understand the effects of environment on anti-malarial phenotype stability. The researcher is using genomic DNA and RNA approaches, including PCR with degenerate primers and microarray analysis of differentially methylated and expressed genomic regions, to identify genes involved in sesquiterpene biosynthesis and develop molecular markers. Progress includes successful PCR amplification, genomic library construction, and initial microarray hybridizations.
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