Myzus persicae is an economically important pest infecting more than 40 families of crops throughout the world. Six entomopathogenic fungal isolates, three each ofBeauveria bassiana and Verticillium lecanii, were screened for pathogenicity test against the M. persicae to select high virulent isolate with the most suitable application and to determine the role of individual enzyme in its virulence. Two treatments that is, conidial shower (190±23 conidia/mm2) and filtrate (3 ml filtrate per treatment from six days liquid broth culture of 1.0x108 conidia ml-1) were conducted for virulence or toxicity test and a comparison was made between treatments and among fungal isolates against the target pest. The percent mortality rates of filtrate at each day, after inoculation was found higher as compared to percent mortality of conidial showering.V. lecanii 3 showed highest virulence or toxicity against the target pest treated either with conidial (80.70%) or filtrate (88.36%) application while B. bassiana 70 and B. bassiana 76 showed high toxicity (77.14 and 80.86%, respectively) in filtrate application at 6th day of incubation. The aphidicial activities of the fungal isolates were evaluated by correlating the enzymes (Chitinase, protease and lipase) activities with enzymes production. On the basis of enzymatic activities, lipase was assumed to participate more in the total virulence or pathogenicity as compared to protease and chitinase while protease was assumed to participate more than chitinase. The pathogenicity test reveals the selection, effective application of most virulent isolate and the role of individual enzyme to develop an alternative control agent against M. persicae.
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CIFOR-ICRAF berfokus pada tantangan-tantangan dan peluang lokal dalam memberikan solusi global untuk hutan, bentang alam, masyarakat, dan Bumi kita
Kami menyediakan bukti-bukti serta solusi untuk mentransformasikan bagaimana lahan dimanfaatkan dan makanan diproduksi: melindungi dan memperbaiki ekosistem, merespons iklim global, malnutrisi, keanekaragaman hayati dan krisis disertifikasi. Ringkasnya, kami berupaya untuk mendukung kehidupan yang lebih baik.
CIFOR–ICRAF achieves science-driven impact. We conduct innovative research, strengthen partners’ capacity and actively engage in dialogue with all stakeholders, bringing the latest insights on forests, trees, landscapes and people to global decision making.
CIFOR–ICRAF publishes over 750 publications every year on agroforestry, forests and climate change, landscape restoration, rights, forest policy and much more – in multiple languages.
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