Rumen protozoa prey on feed-degrading bacteria synthesizing microbial protein, lowering nitrogen utilization efficiency in ruminants. In this in vitro study, we evaluated six plants (Adansonia digitata, Flemingia macrophylla, Kalimeris indica,Brassica rapa subsp. chinensis, Portulaca oleracea, and Calotropis gigantea) for their potential to inhibit rumen protozoa and identified the phytochemicals potentially responsible for protozoa inhibition. Rumen protozoa were anaerobically cultured in vitro in the presence of each plant at four doses. All of the tested plants reduced total rumen protozoa (p ≤ 0.05), but C. gigantea and B. rapa were the most inhibitory, inhibiting rumen protozoa by 45.6 and 65.7%, respectively, at the dose of 1.1 mg/mL. Scanning electron microscopy revealed a disruption of the extracellular structure of protozoa cells. Only C. gigantea also decreased the wasteful ammoniagenesis (p ≤ 0.05). Moreover, the A. digitata extract inhibited α-glucosidase activity by about 70% at 100 µg/mL. Reversed-phase high-performance liquid chromatography analysis detected quercetin, anthraquinone, 3-hydroxybenzoic acid, astragaloside, and myricetin in the tested plant leaves. These plants may hold potential as feed additives to reduce rumen protozoa and α- glucosidase activity. Future research is needed to identify the specific anti-protozoal compound(s), the effects on the rumen microbiome, and its fermentation characteristics.
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CIFOR-ICRAF menerbitkan lebih dari 750 publikasi setiap tahunnya mengenai agroforestri, hutan dan perubahan iklim, restorasi bentang alam, pemenuhan hak-hak, kebijakan hutan dan masih banyak lagi – juga tersedia dalam berbagai bahasa..
CIFOR-ICRAF mendukung dampak ilmiah. Kami melakukan penelitian inovatif, memperkuat kapasitas mitra dan secara aktif terlibat dalam dialog dengan para pemangku kepentingan, menyajikan wawasan termutakhir terkait hutan, pohon, bentang alam, dan masyarakat untuk pengambilan keputusan global.
CIFOR-ICRAF berfokus pada tantangan-tantangan dan peluang lokal dalam memberikan solusi global untuk hutan, bentang alam, masyarakat, dan Bumi kita
Kami menyediakan bukti-bukti serta solusi untuk mentransformasikan bagaimana lahan dimanfaatkan dan makanan diproduksi: melindungi dan memperbaiki ekosistem, merespons iklim global, malnutrisi, keanekaragaman hayati dan krisis disertifikasi. Ringkasnya, kami berupaya untuk mendukung kehidupan yang lebih baik.
CIFOR–ICRAF achieves science-driven impact. We conduct innovative research, strengthen partners’ capacity and actively engage in dialogue with all stakeholders, bringing the latest insights on forests, trees, landscapes and people to global decision making.
CIFOR–ICRAF publishes over 750 publications every year on agroforestry, forests and climate change, landscape restoration, rights, forest policy and much more – in multiple languages.
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